Micronit’s Teardrop, Swirl and Double F glass micromixers are general-purpose chips used across chemistry and nanoparticle applications. Using one for LNP formulation means building a fluidic setup around it with a chip holder, tubing and syringe pumps.

The LNP Screening Array is built for LNP formulation screening and runs on the liquid handler already in the lab. Each LNP Screening Array holds eight micromixers in a standard SLAS deck position, and the liquid handler drives every formulation through a sealed pipette interface.

Micronit’s product pages publish no LNP protocol or characterization data for these chips. Labs formulatingLNPs on them first have to develop their own mixing parameters and characterization methods before they can screen a lipid library.

The LNP Screening Array has published characterization data for blank LNPs made with SM-102, the ionizable lipid in an approved mRNA vaccine, across five liquid handler platforms. On those platforms, blank SM-102 LNPs measured below 60 nm Z-average diameter and below 0.15 PDI by DLS on a Malvern Zetasizer, with Z-average variability below 3% CV.

Each Micronit chip runs in the Fluidic Connect Pro holder. Micronit specifies its own proprietary ferrules and tubing for the holder and warns that other fittings risk leaks or chip damage. Flow comes from separate syringe pumps, which Micronit sells in a starter kit with the holder and chips.

The LNP Screening Array sits in a downholder in a standard deck position and connects through standard pipette tips on a liquid handler, so there is no tubing, ferrule or pump setup required.

A Micronit glass chip is reused across a screen, so every changeover needs a cleaning step. Micronit’s cleaning guidance for its glass chips includes a 1 M sodium hydroxide flush, an ultrasonic bath and heating to about 400°C.

On the LNP Screening Array, each formulation runs through its own micromixer, and the liquid handler starts the next formulation as soon as the previous one is collected.

Micronit offers purpose-built LNP mixers only as custom design and prototyping projects. A lab using the general-purpose chips assembles the holder, fittings, tubing and pumps and develops its own LNP process around them.

The LNP Screening Array comes with an automation protocol configured for your liquid handler, and our applications team installs it and runs the first screen with you on site. LNP Screening Arrays have a three-day lead time.

Talk with our team about the lipid library you want to screen and the liquid handler your lab runs today.